AirLocalize: A Software to Localize Single Molecules in 2D and 3D Images.
Lionnet et al Nature Methods 2011
Airlocalize combines a Gaussian Kernel with a local affine background subtraction to calculate the position and brightness of each particle in the image. Can process individual images, series of images, movies.
Airlocalize is the basis of the EASI-FISH pipeline (Biorxiv / Github)